diff --git a/target_if/init_deinit/src/init_event_handler.c b/target_if/init_deinit/src/init_event_handler.c
index ebda652023..96cc5b3a56 100644
--- a/target_if/init_deinit/src/init_event_handler.c
+++ b/target_if/init_deinit/src/init_event_handler.c
@@ -224,33 +224,6 @@ static inline void init_deinit_update_tdls_caps(struct wmi_unified *wmi_handle,
 {}
 #endif
 
-#ifdef WLAN_MLO_MULTI_CHIP
-static void init_deinit_mlo_tsf_sync_support(struct wmi_unified *wmi_handle,
-					     struct wlan_objmgr_psoc *psoc)
-{
-	uint8_t ml_grp_id;
-	struct mlo_mgr_context *mlo_ctx = wlan_objmgr_get_mlo_ctx();
-	struct mlo_setup_info *mlo_setup;
-	bool mlo_tsf_sync_enab = false;
-
-	ml_grp_id = wlan_mlo_get_psoc_group_id(psoc);
-	if (ml_grp_id < 0) {
-		target_if_err("Invalid ML Grp ID");
-		return;
-	}
-
-	mlo_setup = &mlo_ctx->setup_info[ml_grp_id];
-	if (wmi_service_enabled(wmi_handle, wmi_service_mlo_tsf_sync))
-		mlo_tsf_sync_enab = true;
-
-	mlo_setup->tsf_sync_enabled &= mlo_tsf_sync_enab;
-}
-#else
-static void init_deinit_mlo_tsf_sync_support(struct wmi_unified *wmi_handle,
-					     struct wlan_objmgr_psoc *psoc)
-{}
-#endif
-
 static int init_deinit_service_ready_event_handler(ol_scn_t scn_handle,
 							uint8_t *event,
 							uint32_t data_len)
@@ -407,8 +380,6 @@ static int init_deinit_service_ready_event_handler(ol_scn_t scn_handle,
 	init_deinit_update_wifi_pos_caps(wmi_handle, psoc);
 	init_deinit_update_tdls_caps(wmi_handle, psoc);
 
-	init_deinit_mlo_tsf_sync_support(wmi_handle, psoc);
-
 	/* override derived value, if it exceeds max peer count */
 	if ((wlan_psoc_get_max_peer_count(psoc) >
 		tgt_hdl->info.wlan_res_cfg.num_active_peers) &&
diff --git a/umac/mlo_mgr/inc/wlan_mlo_mgr_public_structs.h b/umac/mlo_mgr/inc/wlan_mlo_mgr_public_structs.h
index 3a2fef331c..7c80545ca2 100644
--- a/umac/mlo_mgr/inc/wlan_mlo_mgr_public_structs.h
+++ b/umac/mlo_mgr/inc/wlan_mlo_mgr_public_structs.h
@@ -147,7 +147,6 @@ struct mlo_chip_info {
  * @event: event for teardown completion
  * @dp_handle: pointer to DP ML context
  * @chip_info: chip specific info of the soc
- * @tsf_sync_enabled: MLO TSF sync is enabled at FW or not
  */
 struct mlo_setup_info {
 	uint8_t ml_grp_id;
@@ -165,7 +164,6 @@ struct mlo_setup_info {
 	qdf_event_t event;
 	struct cdp_mlo_ctxt *dp_handle;
 	struct mlo_chip_info chip_info;
-	bool tsf_sync_enabled;
 };
 
 /**
diff --git a/umac/mlo_mgr/src/wlan_mlo_mgr_setup.c b/umac/mlo_mgr/src/wlan_mlo_mgr_setup.c
index c903edc2c6..c153a53d20 100644
--- a/umac/mlo_mgr/src/wlan_mlo_mgr_setup.c
+++ b/umac/mlo_mgr/src/wlan_mlo_mgr_setup.c
@@ -434,7 +434,6 @@ void mlo_setup_init(uint8_t total_grp)
 	mlo_ctx->setup_info = setup_info;
 	mlo_ctx->setup_info[0].ml_grp_id = 0;
 	for (id = 0; id < total_grp; id++) {
-		mlo_ctx->setup_info[id].tsf_sync_enabled = true;
 		if (qdf_event_create(&mlo_ctx->setup_info[id].event) !=
 							QDF_STATUS_SUCCESS)
 			mlo_err("Unable to create teardown event");
diff --git a/wmi/inc/wmi_unified_param.h b/wmi/inc/wmi_unified_param.h
index f4aeb43440..9b24187854 100644
--- a/wmi/inc/wmi_unified_param.h
+++ b/wmi/inc/wmi_unified_param.h
@@ -6344,9 +6344,6 @@ typedef enum {
 	wmi_service_pre_rx_timeout,
 #ifdef QCA_STANDALONE_SOUNDING_TRIGGER
 	wmi_service_standalone_sound,
-#endif
-#ifdef WLAN_FEATURE_11BE_MLO
-	wmi_service_mlo_tsf_sync,
 #endif
 	wmi_services_max,
 } wmi_conv_service_ids;
diff --git a/wmi/src/wmi_unified_tlv.c b/wmi/src/wmi_unified_tlv.c
index 666b89ae0e..510b00201b 100644
--- a/wmi/src/wmi_unified_tlv.c
+++ b/wmi/src/wmi_unified_tlv.c
@@ -22222,9 +22222,6 @@ static void populate_tlv_service(uint32_t *wmi_service)
 	wmi_service[wmi_service_standalone_sound] =
 			WMI_SERVICE_STANDALONE_SOUND;
 #endif
-#ifdef WLAN_FEATURE_11BE_MLO
-	wmi_service[wmi_service_mlo_tsf_sync] = WMI_SERVICE_MLO_TSF_SYNC;
-#endif
 }
 
 /**